EvALUATION OF FACTORS INFLUENCING THE ANALYSIS

ERS HelenW.Belding,M.D., Winston-Salem.N.C. Conrad Maier, M.D., Zurich, Switzerland Sheila T. Callender, M.D., Oxford, England Milo Xetou ek,M.D., Praha, Czechoslovakia Robert B. Chodos, M.D., Franninghain, Mass. Charles E. Rath, M.D., Washington Roger C. Crafts, Ph.D., Cincinnati Jean P. Soulier, M.D., Paris, France C. R. DasGupta, M.D ., Calcutta, India Ram#{243}n M. Su#{225}rez,M.D.,SanJuan, Puerto Rico Solomon Estren, M.D., New York Timothy R. Talbot, Jr., M.D., Philadelphia Oliver P. Jones, Ph.D., Buffalo Philip F. Wagley, M.D., Baltimore Jan WaldenstrOm, M.D., Upsala, Sweden ERYTHROCYTE MORPHOLOGY and PHYSIOLOGY THE INCORPORATION OF ALPHA-C’4-ACETATE INTO THE STR0SIA OF THE ERYTUROCYTE. K. I. Altman, R. N. Walman and K. Salomon. From the Unsiversitv of Rochester, School of Medicine and Dentistry, Rochester, N. Y. Arch. Biochem. arid! Bio )hys. 33: 168169, 1951. The authors report that acetate is rapidly incorporated! into the stronssa of the erythsrocvte and that there is a rapid turnover of the C’4-labeled constituents of the stroma. This is interpreted to mean that, unlike the circulating red cell hemoglobils, the labeled st-roma components are metabolically active and perhaps exchange with chsenucally identical plasma constituents. It is noted that the highest isotope concentrations in stronsa were observed in a splenectomized rabbit, suggesting that the catabolic phase of stronna metabolism is altered. These studies were carried out ins connsection with investigation of ans ansemia 1)roduced ins the rabbit by ligations of the splenic and gastric coronary veins.-(’.E.R. MECHANICAL FRAGILITY OF THALASSAEMIC ERYTHROCYTES. P. Tolentino. Frons the I)epartment of Pediatrics, University of Genoa, Italy. Londois, Nature 167: 905, 1951. The in vitro mechanical fragility of tens normal and tens thalassemic samples of blood was tested by shaking the blood with steel globules for 20 minutes in horizontal tubes in a Kahn shaker, specimens being removed periodically for red cell counst anid photometric (!etermination of solubilized hemoglobin. The results insdicate that thalassensic erythrocytes show decreased fragility to in vitro traumatization.-S.T.C. IN Vivo SURVIVAL OF RABBIT’S ItEm) CELLS RECOVERED AFTER FREEZING. H. A . Slon’iter. From the National Institute for Medical Research, Mill Hill, London, Ensglansd. Lancet 1: 1350-1351, 1951. Rabbit re(I blood cells were recovered! after freezing ins the presensce of glycerol ansd! storage at -79 C. for 2 hours, 20 !ays and! 42 (lays. They were labeled ins vitro with either 32 or Cr”. Samples were injected into the rabbitfrom which the blood! hsad originsally beens taken and! the radioactivity remaining in the circulating cells at intervals after injections was compared with that found after inject-ion of unstored, unsfrozens labeled cells. With this method!, itwas possible to obtain insformation about survival of red cells for it few days only, but the results indicate thatduring this period the survival of the control and the 1)reviously frozen red cells was similar.-S.T.(’. THE RELATIONSHIP OF THE ARTERIAL OXYGEN SATuRATION TO CYANOSIS. J. E. Geraci and E. H. Wood. From the Division of Medicine, Mayo Clinic, Rochester, Minni. M. Clin. North America. 1185-1202, 1951. This article is of general interest. It points outthat cyanosis is nsot usually recognized until the arterial oxygen saturation values fall to about 75 per cent-. Othier interest insg data are given concerning the relationship of reduced hemoglobins content to cyanosis.T.R.T., Jr. For personal use only. on October 21, 2017. by guest www.bloodjournal.org From